Analyzing The Interactions Of Kdm5/lid And Sin3 In Drosophila Melanogaster
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ANALYZING THE INTERACTIONS OF KDM5/LID AND SIN3 IN DROSOPHILAMELANOGASTERbyAMBIKAI GAJANMay 2015Advisor:Dr. Lori A. PileMajor:Biological SciencesDegree:Doctor of Philosophy SIN3, the scaffold protein of a histone modifying complex is conserved fromyeast to mammals. Drosophila SIN3 associates with both a histone deacetylase RPD3and a histone demethylase dKDM5/LID. Immunopurification of dKDM5/LID verifies apreviously observed interaction with SIN3 and RPD3. Furthermore, deficiency ofdKDM5/LID phenocopies deficiency of SIN3 in many cellular and developmentalprocesses. Knockdown of both Sin3A and lid hinder cell proliferation in Drosophilacultured cells and developing flies. Knockdown of these genes also results in a curvedwing phenotype implicating a role in wing development. Analysis of underlying geneexpression changes upon decreased expression of SIN3, dKDM5/LID or both at agenome wide level determined multiple genes that are commonly regulated by SIN3and dKDM5/LID. Common gene targets of SIN3 and dKDM5/LID are implicated inprocesses related to stress tolerance. Additive roles of these proteins seem important inthe regulation of cell cycle associated genes. Induction of paraquat mediated oxidativestress found a higher number of genes to be regulated by both SIN3 and dKDM5/LID,
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Kdm5/Lid Regulates Chromosome Architecture in Meiotic Prophase I Independently of Its Histone Demethylase Activity
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